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Construction of genetically engineered strains of Enterobacter cloacae (nifL~(-)A~(c)) by gene targeting mutation

Qiu Deyi, Shen Bingfu

Shanghai Institute of Plant Physiology,Chinese Academy of Scioences.Shanghai 200032

Abstract

E. cloacae E26 nifL mutation in chromosome was constructed by in vitro insertion of Tc~(r) gene into the Sma I site located in the nifL 3^-terminus f plasmid pST1142 and by in vivo homologous DNA recombination. Recombinants (E12 and E13) were obtained by selection, both the Tc~(r) gene inserted in the chromosome nifL in the opposite transcription orientation. A promoter-like nucleotide sequence (-TTTCATA-) was produced in the upstream region of the nifA in E13.

Key words: Gene expression;nifL;nifA;Enterobacter cloacae

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Citing This Article：

Qiu Deyi, Shen Bingfu. Construction of genetically engineered strains of Enterobacter cloacae (nifL~(-)A~(c)) by gene targeting mutation. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).