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Expression of cyclin dependent kinase inhibitor p27 during proliferation in vascular smooth muscle cell

Yuan Yong, Xu Dingli, Liu Yili, Jia Manying

Department of Cardiology,Nanfang Hospital,The First Military Medical University.Guangzhou 510515,Guangdong

Abstract

Deprived of fotal calf serum for 48 h, cultured VSMCs in quiescent condition were collected at different times after stimulation of AngⅡ, AVP and PDGF-BB. Cell cycle distribution and p27 expression were determined with a flow cytometer. The results showed that the protein content of VSMCs was significantly increased (43.6%) by AngⅡ as a result of hypertrophy, but AngⅡ did not lead to downregulation of p27. AVP could downregulate p27 slightly. PDGF could inhibit p27 expression significantly and cause VSMCs hyperplasia.

Key words: p27;Cell cycle;Vascular smooth muscle cell;Flow cytometer

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Citing This Article：

Yuan Yong, Xu Dingli, Liu Yili, Jia Manying. Expression of cyclin dependent kinase inhibitor p27 during proliferation in vascular smooth muscle cell. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).