Issue Archive

Expression of human superoxide dismutase gene in rat vascular smooth muscle cells and its anti-oxidative effect

Zheng Xincheng, An Wei, Bai Jingxiang, Mao Songhua, Wu Yijing

Department of Pathophysiology,Beijing Medical University.Beijing 100083；China；Department of General Surgery,The People^s Hospital of Beijing Medical University.Beijing 100035

Abstract

A retroviral vector containing human superoxide dismutase (SOD) cDNA was constructed and transfected into rat vascular smooth muscle cells (VSMCs). The expression of exogenous hSOD_(1) in the VSMCs was analyzed with Northern and Southern blot. The protection of the transfected and/or non-transfected VSMCs from free radical produced by the xanthine/xanthine oxidase (X/XO) system was investigated. It is suggested that the expression of gene-transferred hSOD_(1) is able to prevent the formation of atherosclerosis, partially due to its cell protection and inhibition of the proliferative embolization.

Key words: Superoxide dismutase (SOD);Gene;Vascular smooth muscle cells;Free radical

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Zheng Xincheng, An Wei, Bai Jingxiang, Mao Songhua, Wu Yijing. Expression of human superoxide dismutase gene in rat vascular smooth muscle cells and its anti-oxidative effect. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).