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Studies on the energy liberation of rice mitochondria under different conditions by means of calorimetry

Zhou Peijiang, Ling Xingyuan, Zhou Hantao, Qu Songsheng, Zhu Yingguo

College of Life Sciences,Wuhan University.Wuhan 430072,Hubei；China

Abstract

This paper reported the thermodynamic and kinetic behaviours of the energy liberation of the mitochondria isolated from Guangchong 41 fertile lines of rice (Oryza sativa L.) studied by using an LKB-2277 bioactivity monitor and a Dupont-910 differential scanning calorimeter (DSC) & 9900 computer/thermal analyzer system under constant and changing temperature. The thermograms of energy liberation of the rice mitochondria (which had been kept at 0-3℃ for 15h and 40d) were determined respectively at 25 and 30℃, and the difference in shape of the thermograms and thermodynamic and kinetic characteristics were compared. The results indicated that the lower the temperature, the slower the energy liberation rate of the rice mitochondria. Both the heat releast and the energy liberation rate of the rice mitochondria increased after the mitochondria had been kept at lower temperature for long time. In this work, authors also determined the DSC curve of the rice mitochondrial energy liberation under changing temperature, and calculated the thermodynamic and thermodynamic and kinetic characteristic parameters of the rice mitochondrial energy liberation under changing temperature.

Key words: Rice(Oryza sativa L.);Mitochondria energy liberation;Calorimetry;Thermodynamics;Kinetics;

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Citing This Article：

Zhou Peijiang, Ling Xingyuan, Zhou Hantao, Qu Songsheng, Zhu Yingguo. Studies on the energy liberation of rice mitochondria under different conditions by means of calorimetry. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).