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Effect of human inhibin #alpha#-fragment 1-32-tyr (P~(33)) on apoptosis of cultured rat corpus luteal cells

Zhang Jianghong, Yu Jin, Feng Youji, Ni Jiang, Cheng Zhiping, Sun Ying, Lu Guichen, Wu Yanwan, Li Weixiong

Institute of Obstetrics/Gynecology,Shanghai Medical University.Shanghai 200011；China；Institute of Materia Medica,Chinese Academy of Sciences.Beijing 100050

Abstract

In the present work, the action of P~(33) on apoptosis was further studied in vitro in cultured rat CL cells. Gel electrophoretic analysis for detection of oligonucleosomal DNA fragmentation, AO-EB or PI assays and flow cytometry were used to observe the action of P~(33) on the occurrence of spontaneous apoptosis by collegenase-DNase dispersed CL cells, obtained from PMSG-hCG induced pseudopregnent rats. The results suggest that P~(33) stimulates spontaneous apoptosis in cultured rat CL cells with the involvement of tyrosine specific protein kinase system.

Key words: Apoptosis;Corpus luteal cells;Inhibin #alpha#-subunit fragment;Tyrosine specific protein kinase (TPK);RNA synthesis;Protein synthesis rat;

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Citing This Article：

Zhang Jianghong, Yu Jin, Feng Youji, Ni Jiang, Cheng Zhiping, Sun Ying, Lu Guichen, Wu Yanwan, Li Weixiong. Effect of human inhibin #alpha#-fragment 1-32-tyr (P~(33)) on apoptosis of cultured rat corpus luteal cells. Acta Physiol Sin 1999; 51 (1): 87-95 (in Chinese with English abstract).