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Effects of glutamic acid and acetylcholine on induction of heat shock proteins 70 mRNA in PC12 cells

Wu Bingyi, Lou Shujie, Xu Renbao, Chen Yizhang

Department of Physiology,Second Military Medical University.Shanghai 200433；China

Abstract

In the present study, the effects of glutamic acid and acetylcholine (ACh) on the induction of hsp70 mRNA in PC12 cells were studied by Northern blot method. The probe used is specific for inducible hsp70 mRNA. Our results showed that the glutamic acid under limited conditions (such as at 50-500#mu#mol/L and action time 5-30min) could induce the expression of hsp70 mRNA, which was partly mediated by NMDA receptors. On the other hand, ACh (0.1-1 000#mu#mol/L) could not induce the expression of hsp 70 mRNA.

Key words: Heat shock proteins;Neurotransmitters;PC12 cells;Glutamic acid;Acetylcholine

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Citing This Article：

Wu Bingyi, Lou Shujie, Xu Renbao, Chen Yizhang. Effects of glutamic acid and acetylcholine on induction of heat shock proteins 70 mRNA in PC12 cells. Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).