Issue Archive

Effect of salt stress on calcium-activated protein kinase associated with plasma membrane from corn (Zea mays L.) root tips

Chen Wu, Chen Jia

College of Biology,China Agricultural University.Beijing 100094

Abstract

NaCl 0.2mol/L treatment could induce quickly the activity of calcium-activated protein kinase, which showed the character of calcium-dependent protein kinase (CDPK), in plasma membrane (PM) from corn root tips. The activity reached its maximum after 15 min of salt stress. Then, the activity decreased gradually, remained higher than the control 50 min after the beginning of salt stress. Treatment with 10% PEG could also increase the activity of calcium-activated protein kinase. However, exogenous ABA did not change the activity of the calcium-activated protein kinase in PM. In gels assays of SDS-PAGE show that the proteins kinase contained only one polypeptide. It was shown by autoradiography that two proteins in PM with molecular weights of 33 kD and 58 kD may be the endogenous substrates of the calcium-activated protein kinase in PM.

Key words: Corn root tips;Plasma membrane;Calcium;Calcium-activated protein kinase;Salt stress;Signal transduction;Calcium-dependent protein knase

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Chen Wu, Chen Jia. Effect of salt stress on calcium-activated protein kinase associated with plasma membrane from corn (Zea mays L.) root tips. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).