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Proteins associated with adaptation of the green alga Dunaliella salina to NaCl stress

Xu Kun, Li Lin, Ouyang Guangcha, Jiao Xinzhi

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032；China

Abstract

SDS-PAGE profiles of cell extracts from the green alga, Dunaliella salina, grown in different salinities revealed that the amount of the 51 kD, 23 kD and 63 kD proteins were more abundant in the alga cells when the concentration of the NaCl in the culture medium was 1 mol/L than when it was 3 mol/L. In contrast, the 23 kD protein was more aboudant in cells grown in culture medium with NaCl 3 mol/L; Its relative amount in cells grown in culture medium with NaCl 3 mol/L or more was about two folds higher than that of cells in culture medium with NaCl 1 mol/L or less. Furthermore, when the cells grown in culture medium with NaCl 1 mol/L were subjected to drastic hyperosmotic shock (NaCl 1 mol/L→3 mol/L), the amount of 23 kD protein increased, while the amount of 51 kD protein decreased. No obvious changes of the amount of the two proteins were observed when the cells grown in culture medium with NaCl 3 mol/L were subjected to hypoosmotic shock→NaCl 3 mol/L→1 mol/L) even after 24h treatment. In addition, an unstable 26 kD protein was easily digested. The possible relationship of these proteins with osmoregulation were analyzed as well.

Key words: Dunaliella salina;Osmotic shock;Osmoregulation

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Citing This Article：

Xu Kun, Li Lin, Ouyang Guangcha, Jiao Xinzhi. Proteins associated with adaptation of the green alga Dunaliella salina to NaCl stress. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).