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Region-directed mutagenesis and sequence analysis of exoL gene encoding a glucosyl-transferase of Rhizobium huakuii

Gong Feng, Li Qiang, Zhou Beiyun, Song Yongyu

Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

The resulting plasmid pJB-B601 could restore the Exo~(-) phenotype of Rh983. Determination of the complete nucleotide sequence of the fragment showed that it contained the structural gene of a glucosyl-transferase, as well as the 5^- and 3^- flanking regions. An open reading frame of 1 170 base pairs was identified as R. huakuii exo L gene. The MW of ExoL, as deduced from the nucleotide sequence, was estimated at 44kD. Comparison of the nucleotide sequences revealed a high similarity between the exoL genes of R. huakuii and of R. meliloti. The deduced amino acid sequence of R. huakuii ExoL also showed a high similarity with that of R. meliloti ExoL. Furthermore, the exoL-lacZ transcription fusion was constructed and the expression of exoL-lacZ was analyzed.

Key words: Rhizobium huakuii;Glucosyl -transferasegene;EPS;DNA sequence

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Citing This Article：

Gong Feng, Li Qiang, Zhou Beiyun, Song Yongyu. Region-directed mutagenesis and sequence analysis of exoL gene encoding a glucosyl-transferase of Rhizobium huakuii. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).