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A simple method of isolation and identification of P cells in rat sinoatrial node

Abstract

A method of isolating P cells of rat sinoatrial node by collagenase is described. The method is feasible for morphological and electrophysiological investigation, with the advantages of taking a relatively easy procedure and consuming a small amount of collagenase. The main features of action potentials of these isolated cells including clear diastolic automatic depolarization, mean maximal diastolic potential of -55mV, mean peak potential value of 58mV, and mean APD_(50) of 18ms, were similar to those of P cells in situ.

Key words: Sinoatrial node;Pacemaking cells;Myocardial cell;Collagenase;Rat

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Citing This Article：

. A simple method of isolation and identification of P cells in rat sinoatrial node. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).