Neural differentation of Wnt-1 overexpression P19 cells
Abstract
Wnt-1 gene plays important roles during the development of the mouse central neural system, and shown a transient expression during neuronal differentiation of P19 EC cells. We transfected Wnt-1 gene into P19 cells and found the Wnt-1 transfected cells could differentiate into neurons without the induction of RA. However, MASH-1, a crucial gene for the early neural differentiation, was expressed later than P19 cells induced by RA.
Key words: Wnt-1;Reverse transcriptional PCR;P19 cells;Overexpression;Neuronal differentiation;MASH-1
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Citing This Article:
. Neural differentation of Wnt-1 overexpression P19 cells. Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).