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Peculiarities of nuclear DNA multiplication during embryogenesis in rice embryo cells

Tang Xihua, Zhang Yinping

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Nuclear DNA content was determined for glubular, differentiating and naturing embryos during rice embryogenesis. Measurment of nulear-DNA was done microspectro-photometrically using late prophase nuclei in Feulgen-DNA stained embryo squashes. The results show that differences in nuclear DNA cotent between embryos at various developmental stages. The nuclei measured in the globular embryo showed DNA values varying from 6 C to 12 C, i.e., they were polyploid. The highest DNA values found in the embryo were 238 C or 439 C. These cells may be located in the s u s pensor of early developing embryos. At later developmental stages of rice embryo, the nuclear DNA values varied from 20 C to 56 C. But the cells in the scutellum had higher C values than those in plumule and in primary radicle. These results of nuclear-DNA measuments for nuclei in glubular embryo as well as in different primordia in differentiated embryos offer strong evidence for differential DNA replication during rice embryogenesis.

Key words: Rice;Embryogenesis;Nuclear-DNA content;Polyploidy;Differential DNA replication

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Citing This Article：

Tang Xihua, Zhang Yinping. Peculiarities of nuclear DNA multiplication during embryogenesis in rice embryo cells. Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).