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Plant factors inducing the expression of nodulation gene in Rhizobium astragali

Gao Bingli, Lou Wuji, Chen Yongxuan, Fan Qingsheng, Gao Chengjiang, Shen Sishi, Jin Runzhi

Microbiology Department,Nanjing Agricultural University.Nanjing 210095,Jiangsu；China

Abstract

Using column chromatography, TLC and HPLC, from Astragalus sinicus seed we isolated and purified the compounds which can induce the expression of nod genes in Rhizobium astragli. An inducer was identified as naringenin by MS. We also tried nineteen standard samples including flavonoids and nonflavonoid to detect their activity in inducing the expression of nod genes in Rhizobium astragali. The results domostrated that the nod genes of Rhizobium astragali can respond to several inducing compounds, i.e. the flavonoids naringenin, lutiolin and daidzeid and nonflavonoids umbelliferone and trigonelline.

Key words: Astragalus sinicus;Flavonoid;Induction;Nod gene

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Citing This Article：

Gao Bingli, Lou Wuji, Chen Yongxuan, Fan Qingsheng, Gao Chengjiang, Shen Sishi, Jin Runzhi. Plant factors inducing the expression of nodulation gene in Rhizobium astragali. Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).