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Immunogold electron microscopy localization of brassinolide in epicotyl cells of mung bean

Abstract

With immunogold (PA-Au complexes) electron microscopy the localization of brassinolide (BR) in epicotyl cells of mung bean (Phaseolus radiatus L.) has been studied. The results showed that, in thin sections treated with anti-BR, choroplasts, nucleoti, vacuoles, starch grains and cell membranes were labelled by gold particles, with choroplasts, nucleoti and vacuoles being labelled more heavity. However, no gold particles were found in cell walls. The sections with no EDC fixation contained very few gold particles, and those treated with preimmune rabbit serum contained much fewer gold particles.

Key words: Mung bean;Epicotyl brassinolide(BR);Immunogold electron microscopy; Localization;

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Citing This Article：

. Immunogold electron microscopy localization of brassinolide in epicotyl cells of mung bean. Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).