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The presence of p5 cs gene in rice and its function in proline-overproducing rice plant lines

Abstract

Hybridization analysis was conducted using proline synthase △^-pyrroline-5-carboxylate synthetase (P5CS) cDNA from root nodule of cowpea as probe. The results indicated the presence of the homologue sequence of p5cs gene of cowpea nodule in rice plant. Its transcription level increased under salt stress. The poline content and salt tolerance of the filial generation (F_(9)) of proline-overproducing rice plant lines were markedly higher than those of the original type. Using these proline-overproducing rice plant lines as experimental materials to conduct hybridization analysis, it has been shown that the high proline content characteristics and increased salt tolerance are correlated to the presence of this gene and its increased transcription.

Key words: Proline;#DELTA#’-pyrroline-5-carboxylate synthetase gene;Salt tolerance;Rice

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Citing This Article：

. The presence of p5 cs gene in rice and its function in proline-overproducing rice plant lines. Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).