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DNA structure analysis of chitinase gene clone RCH8 from rice

Li Hong, Zhu Qun, Bai Yongyan

Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032；USA

Abstract

RCH8 is a basic chitinase genomic clone isolated from rice (var. IR36) genomic library using a bean chitinase gene fragment as a probe. The complete nucleotide sequence was determined in both directions method. Deletions of the DNA chain for sequencing were created by exonuclease Ⅲ and S1 nuclease. The 2049bp clone contains a 1057bp upstream sequence and a single, complete open reading frame of 966bp with no introns. The 5^ side sequence contains possible regulatory elements. The ORF encodes a polypeptide of 322 amino acids with a putative 20 amino acid N-terminal signal peptide followed by a 40 amino acid cysteine-rich domain containing 8 cystein residues and a chitinase catalytic domain. The deduced amino acid sequence shares 88.5%, 80.4% and 78.9% identity with those of three other published basic chitinase genes from rice. In addition, the hevein domain sequence homologies of nucleic acids and amino acids were compared between RCH8 and several other proteins.

Key words: Rice (Oryza sativa L. cv. IR36);Chitinase gene clone;DNA sequence and analysis

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Citing This Article：

Li Hong, Zhu Qun, Bai Yongyan. DNA structure analysis of chitinase gene clone RCH8 from rice. Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).