Cloning of an unlinked exo site from EPS synthesis deficient mutants of Rhizobium astragali strain 107
Zeng Weiqing, Song Hongyu
Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032
Abstract
By complementation test of NA-05, NA-07 and NA-08 with plasmids carrying exo genes of R. meliloti and Rhizobium sp. NGR234, the function of the exo gene cloned in pRK415 (the pRKM578) was identified as encoding for UDP-glucose-4-epimerase, as the exoB of R. meliloti and exoC of R. sp. NGR234.
Key words: Shtgun method;Rhizobium astragali;Exo gene cloning;Physical mapping
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Citing This Article:
Zeng Weiqing, Song Hongyu. Cloning of an unlinked exo site from EPS synthesis deficient mutants of Rhizobium astragali strain 107. Acta Physiol Sin 1997; 49 (4): (in Chinese with English abstract).