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Role of Ca~(2+) and calmodulin on freezing tolerance of citrus protoplasts

Li Wei, Sun Zhonghai, Zhang Wencai, Ma Xiangtao, Liu Daohong

Department of Life Science,Hubei University.Wuhan 430062,Hubei；China

Abstract

Ca~(2+) and CaM plays important role in the acquistition of freezing tolerance by citrus protoplasts during cold acclimation. Ca~(2+) chelation or antagonism to calmodulin markedly inhibited the development of cold-induced freezing tolerance in protoplasts. Calcium ionophore A_(23187) strongly increased freezing tolerance of the protoplasts without cold acclimation, and the effects of A_(23187) was weakened by TFP, a calmodulin antagonist. These results suggest that freezing tolerance of protoplasts was mediated by Ca~(2+) and calmodulin.

Key words: Protoplast;Freezing-tolerance;Calcium;Calmodulin;Citrus

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Citing This Article：

Li Wei, Sun Zhonghai, Zhang Wencai, Ma Xiangtao, Liu Daohong. Role of Ca~(2+) and calmodulin on freezing tolerance of citrus protoplasts. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).