Selection of brassinolide-insensitive mutant of Arabidopsis thaliana
Zheng Huiqiong, Chen Jichu, Zhao Yuju, Xu Zhihong
Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032
Abstract
Arabidopsis thaliana mutants insensitive to brassinolide (BR) were isolated by screening for growth of seedling on a medium containing epi-BR 0.05mg/L. The mutant phenotypically changed in the morphology of roots and leaves. Genetic analysis of these mutants indicated that the BR insensitivity was due to a recessive mutation, which was designated as br. In addition, studies on the effects of epi-BR on the growth of leaves of mutant progenies and pH change of the bathing medium by using leaf discs showed that leaves of the mutant were as insensitive to epi-BR as roots.
Key words: Arabidopsis thaliana;Brassinolide;Insensitive-mutunt
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Citing This Article:
Zheng Huiqiong, Chen Jichu, Zhao Yuju, Xu Zhihong. Selection of brassinolide-insensitive mutant of Arabidopsis thaliana. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).