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A regulation-related intron in 5^ untranslated region of rice waxy gene

Cai Xiuling, Wang Zongyang, Zheng Feiqin, Hong Mengmin

Shanghai Institute of Plant Physiology,The Chinese Academy of Science.Shanghai 200032

Abstract

Using the methods of polymerase chain reaction-amplified reverse-transcribed RNA isolated from the developing seeds and sequence analysis of amplified product, an intron of 1126 bp in size was identified in the 5^-untranslated region of rice Oryza sativa subsp. indica 232 cultivar Wx gene (Figs 2, 3). Its A+T content was found to be 67.4% (Table 1). The border sequences of this intron conform to the GT-AG rule. Considering similar results of previous experiments, it is suggested that this intron may play an important role in the regulation of Wx gene expression.

Key words: Nitron 1;Wx gene;Rice

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Citing This Article：

Cai Xiuling, Wang Zongyang, Zheng Feiqin, Hong Mengmin. A regulation-related intron in 5^ untranslated region of rice waxy gene. Acta Physiol Sin 1997; 49 (3): (in Chinese with English abstract).