Issue Archive

Resistant characteristics of the hydroxyproline resistant cell line in Onobrychis viciaefolia after protoplast culture

Xu Ziqin, Jia Jingfen

Cell Biology Laboratory of Lanzhou University. Lanzhou 730000, Gansu

Abstract

The hydroxyproline resistant cell line of Onobrychis viciaefolia had been　selected through NaN_(3) mutagenesis. Protoplast-derived plantlets of the　resistant cell line were regenerated in four months and were found to retain　strong hydroxyproline resistance. After the calli of the resistant cell line　derived from protoplasts were treated with different concentrations of NaCl,Hyp or PEG, the free proline content increased steeply in the first week and　descended to normal level in three weeks. In contrast to this, proline　accumulation in the wild tissue was not found at low selection level it could　stand. Along with the enhancement of stress, the free proline concentration in　calli of the resistant cell line increased, while the relative growth rate　descended. When the resistant cell line was transferred from selection medium onto normal MS medium, its free proline content declined　to the normal level in six days. It suggested that free proline　accumulation was related to osmotic regulation and was the major way to　prevent water loss of the resistant cell line under water stress. Total amino　acid analysis revealed that methionine content of the regenerated plantlet　leaves derived from hydroxyproline-resistant protoplasts was twice as high as the original type.

Key words: Onobrychis viciaefolia;Hydroxyproline;Free proline;Protoplast;Resistance;Stable inherity;Osmotic regulation

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Xu Ziqin, Jia Jingfen. Resistant characteristics of the hydroxyproline resistant cell line in Onobrychis viciaefolia after protoplast culture. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).