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Cloning of auxin binding protein cDNA and its expression in tobacco

Zhang Hongxia, Bai Yongyan, Xu Zhihong

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai 200032

Abstract

According to the reported coding sequence of auxin binding protein(ABP) located in the endoplasmic reticulum of Arabidopsis, we have designed and synthesized two primers Ap5 and Ap3. The whole sequence of ABP cDNA was cloned by RT-PCR and confirmed by DNA sequencing.Plant expression vector p35SE was constructed to transform the explants of tobacco(SR1) leaves by Agrobacterium-mediated method.Southern and Northern analysis confirmed that the foreign ABP gene has been expressed in tobacco plant cells. It was also found that the progenies of p35SE transgenic tobacco plants were significantly auxin-sensitive.

Key words: Auxin binding protein;RT-plymerase chain reaction;Transgenic plant

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Citing This Article：

Zhang Hongxia, Bai Yongyan, Xu Zhihong. Cloning of auxin binding protein cDNA and its expression in tobacco. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).