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Role of cytosolic calcium release on gastrin induced contraction of gastric muscle cells of rat

Zhou Lu, Luo Jiexin

Department of Physiology, Institute of Basic Medical Sciences, CAMS and School of Basic Medicine, PUMC. Beijing 100005

Abstract

The present study was conducted to investigate the contractile effect of pentagastrin (G5) on isolated smooth muscle cells of rat stomach and role of cytosolic-free calcium ([Ca~(2+)]_(i)) release during contraction of gastric muscle cells induceed by G5. The results were as follows: (1) G5 induces contraction of smooth muscle cells isolated from gastric body, anturm and pylorus, in a dose-dependent manner from 4×10~(-8) mol/L to 16×10~(-8)mol/L, with the maximal effectiveness in antrum. (2) The action of G5 can be blocked by proglumide and anti-gastrin serium, but not by atropine. (3) The effect of acetylcholine and G5 are adtive when administrated simulataneously (4) Inhibitor of intracellular Ca~(2+) release TMB-8 could also suppress the G5 effect. (5) The effect of G5 could be seen accompaning significant increase of intracellular Ca~(2+) concentration.

Key words: Isolated gastric smooth muscle;Pentagastrin;Contraction;Free calcium

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Citing This Article：

Zhou Lu, Luo Jiexin. Role of cytosolic calcium release on gastrin induced contraction of gastric muscle cells of rat. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).