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Ischemic preconditional protection of ischemia reperfusion injury on isolated hearts of ground squirrel and rat

Gao Tianli, Huang Yuzhi

College of Life Sciences, Peking University. Beijing 100871

Abstract

The protective effects of ischemic precoditioning on ischemia-reperfusion injury was investigated using isolated Langendorff perfusing hearts from ground squirrel and rat. In Preconditioning I group hearts were first perfused wit Krebs-Henseleit solution for 10 min to establish a steady state, then stopped for 15 min to establish global ischemia, and finally followed by 10 min ischemia and 10 min reperfusion. In Preconditioning Ⅱ group there were three cycles of 5 min ischemia +5 min reperfusion after 10 min equilibration and then the final 10 min ischemia and 10 min reperfusion were followed. It was found that in group Ⅰ durign the final 10 min ischemic period there was remarkable augmentation of CK release from both animal^s hearts. But in group Ⅱ CK release decreased markedly during the same ischemic period. CK release during final 10 min reperfusion period also decreased significantly in group Ⅱ in comparison with group Ⅰ. The incidence of arrhythmias occurred in both animal^s hearts was markedly reduced in group Ⅱ rather than group Ⅰ.

Key words: Ischemic preconditioning;Creatine kinase;Ischemic injury;Reperfusion injury

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Citing This Article：

Gao Tianli, Huang Yuzhi. Ischemic preconditional protection of ischemia reperfusion injury on isolated hearts of ground squirrel and rat. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).