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Effect of interleukin-2 on the proliferation of cultured rat anterior pituitary cells

Wang Gaofeng, Zhu Yunlong, Hu Yuzhen, Zhang Wanhui

The Fouth Military Medical University. Xi^an 710032, Shaanxi

Abstract

The present study was to investigate whether IL-2 is involved in the proliferation control of anterior pituitary (AP) cells. Primary culture of AP cells was obtained from either male or female Sprague-Dawley rats. The effects of IL-2 were assessed by ~(3)Hthymidine incorporation rate. It was found that: (1) IL-2 (10-500 U/ml) significantly stimulated ~(3)H-TdR incorporation into AP cells of female pregnant as well as nont-pregnant rats, but inhibited ~(3)H-TdR incorporation in the male rats. (2) The stimulatory effect of IL-2 was reversed in overiectomized rats, but could be restored when the overiectomized rats were treated with estradiol benzoat (5 #mu#g/d per rat). (3) When the male rats were orchiectomized for 2 weeks, the inhibitory effects of IL-2 did not occur.

Key words: Interleukin-2;Anterior pituitary;Cell growth;3H-TdR;Incorporation estradiol;Rat

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Citing This Article：

Wang Gaofeng, Zhu Yunlong, Hu Yuzhen, Zhang Wanhui. Effect of interleukin-2 on the proliferation of cultured rat anterior pituitary cells. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).