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The effects of gastrin-releasing peptide (GRP) and vasoactive intestinal polypeptide (VIP) on the prolactin (PRL) gene transcription of pitutitary PRL releasing tumor of rat

Di Anke, Shan Huimin, Huang Manying, Xu Rongkun

CMB Visiting Scholar from Department of Physiology, Dalian Medical University.Dalian 116023,Liaoning；China

Abstract

The effects of gastrin-releasing peptide (GRP) and vasoactive intestinal polypeptide (VIP) on the prolacitn gene transcription of cultured pituitary of male Sprague-Dawley (SD) rats PRL releasing tumor (PPRT) (induced by estradiol) cells were studied. The PRL mRNA levels were determined by in situ hybridization of cytoplasmic RNA with a DIG-labeled PRL cDNA probe. PRl mRNA levels didn^t change when the PPRT cells were incubated with 10~(-8) mol/L or 10~(-7) mol/L GRP for 24 h, but decreased by 20% when GRP was increased to 10~(-6) mol/L (P＜0.05).The PRL mRNA level increased to 1.60,2.10,2.21 times of the control group when the PPRT cells were respectively incubated with 10~(-8),10~(-7),10~(-6) mol/L VIP for 24 h (P＜0.05). The PRL mRNA level didn^t change when the PPRT tumor cells were incubatcd with 10~(-8) mol/L E~(2) for 48 h, but did increase to 2.80 and 2.92 times of the control group respectively when 10~(-7) mol/L 10~(-6) mol/L E~(2) were used. The results above indicated that GRP and VIP exert an inhibitory and a stimulatory effect on RPL gene transcription respectively, while the stimulatory action of E~(2) on PRL secretion is a direct one.

Key words: Gastrin-releasing peptide;Vasoactive intestinal polypeptide;Estradiol;Pituitary PRL releasing tumor;Cell culture;In situ hybridhatlon

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Citing This Article：

Di Anke, Shan Huimin, Huang Manying, Xu Rongkun. The effects of gastrin-releasing peptide (GRP) and vasoactive intestinal polypeptide (VIP) on the prolactin (PRL) gene transcription of pitutitary PRL releasing tumor of rat. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).