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Absence of point mutation in the third intracellular loop of #alpha#_(1B)-and #alpha#_(1D) adrenergic receptor in the aortae of spontaneously hypertensive rats

Xu Kaming, Han Qide

Institute of Vascular Medicine, Third Hospital, Beijing Medical University.Beijing 100083

Abstract

According to the constitutive activity hypothesis for G-protein coupling receptors, we inferred that point mutations might possibly exsit in the third intracellular loop of #alpha#_(1B)-and/or #alpha#_(1D)-adrenergic receptor (AR), the main subtypes of #alpha#_(1)-AR mediating phosphatidylinositol hydrolysis in SHR aortae. This inference, however, was not demonstrated by the results of our PCR-SSCT analyses, which indicated that the increased basallevel of IPs accumulation in the isolated aortae from SHR does not result from constitutive activity of #alpha#_(1B)-and #alpha#_(1D)-AR induced by ponit mutations of the third intracellular loop of the genes

Key words: Hypertension;L-Arginine;Smooth muscle cells;Liposome

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Citing This Article：

Xu Kaming, Han Qide. Absence of point mutation in the third intracellular loop of #alpha#_(1B)-and #alpha#_(1D) adrenergic receptor in the aortae of spontaneously hypertensive rats. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).