ISSN 0371-0874, CN 31-1352/Q

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Voltage-dependent potassium currents in rat C_(6) glioma cells

He Shufang, Xie Zhong

Shanghai Brain Research Institute, Chinese Academy of Sciences. Shanghai 200031

Abstract

In the present study, the properties of voltage-dependent K~(+) currents in cultured rat C_(6) glioma cells were investigated with the whole cell patch-clamp recording technique. A total of 97 cells were recorded. The mean value of the membrane potential was -47.5±9.4 mV. Seven percent of the reocrded C_(6) glioma cells did not show any outward potassium currents. The rest had outward K~(+) currents which could be activated at -36.5±5.9 mV from a holding potential of -70 mV. Ananalysis of tail currents, showed the mean value of the reversal potential was -84.6±4.1 mV, close to the K~(+) equilibrium potential. The K~(+) currents of 60% of the recorded C_(6) cells consisted of both a fast and a slow component, while those of the 33% only with a slow component.

Key words: C_(6) glioma cells;K-+ current;Whole-cell petch-clamp recording

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Citing This Article:

He Shufang, Xie Zhong. Voltage-dependent potassium currents in rat C_(6) glioma cells. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).