Issue Archive

Isolation and identification of germination inhibitors in American ginseng fruit

Huang Yaoge, Li Xianggao, Cui Shuyu, Zheng Yinan, Han Baorui

College of Chinese Medicinal Materials, Jilin Agricultural University. Changchun 130118, Jilin

Abstract

The traditional method of germination inhibitor extraction, isolation and identification was improved in the present study. The inhibitors were preliminarily isolated by using a series of solvents,then separated and purified by TLC, and then further isolated and identified with GC-MS. Finally, the IC_(50)(concentration of 50% inhibition) of the inhibitors were determined by calibration with the standards. Three kinds of inhibitor were extractred, isolated and identified for the first time, from the fruit of American ginseng ( Panax quinquefolium L.). They are chloral hydrate, hexanoic acid and octanoic acid and the IC_(50) are 577.6 #mu#l/L, 128.4 #mu#l/L and 87.7 #mu#l/L, respectively.

Key words: American ginseng (Panax quinquefolium L);Fruit;Inhibitors;Chloral hydrate;Hexanoic acid;Octanoic acid

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Huang Yaoge, Li Xianggao, Cui Shuyu, Zheng Yinan, Han Baorui. Isolation and identification of germination inhibitors in American ginseng fruit. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).