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Effect of pollination on protein and Ca~(2+) concentration in stigma and style of rice(Oryza sativa)

Li Xinli, Huang Qingliu, Tang Xihua

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences.Shanghai 200032

Abstract

Five peptides with similar electrophoretic characterisitics belonging to two kinds have been identified in rice stigma and style using NEPHGE and 2-D electrophoresis. In self- or cross-compatible pollinated stigma and style,these peptides without distingwished changes.While a distinct peptide with MW 17.5 kD,pI 8.5 was identified after cross-incompatible pollination,which may be related to the recognition of incompatible pollen.A high concentration of Ca~(2+) was found on the surface of mature stigma,but not on the surface of immature one.Forced pollination induced　concentration of Ca~(2+) to the surface of immature stigma.This suggests that Ca~(2+) be not only related to the maturation of stigma,but also to its contact with pollen.

Key words: Rice (Oryza sativa);Cross-incompatibily;Two-dimensional electrophoresis;Forcing pollination;Peptide;Stigma.;Ca~(2+)

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Citing This Article：

Li Xinli, Huang Qingliu, Tang Xihua. Effect of pollination on protein and Ca~(2+) concentration in stigma and style of rice(Oryza sativa). Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).