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The effects of 1-aminocyclopropane-carboxylic acid and inositol-1,4,5-trisphosphate on cytoplasmic calcium concentration during Chinese rose petal development

Song Chunpeng, Cao Gengsheng, An Guoyong, Ma Xiaodong, Du Zuliang, Zhu Ziqiang, Yan Longfei

Department of Biology, Henan University. Kaifeng 475001,Henan； China；College of Biological Sciences, Beijing Agricultural University. Beijing 100094

Abstract

Protoplasts from Chinese rose(Rosa chinensis Jack cv． Zirong)petals at different development stages were loaded with fura 2 AM to measure Ca~(2+)_(c). The fluorescence of frua 2 was measured at 450 nm using a Spex F212 Fluorolog with excitation at either 340 nm or 370　nm. Fluorescence ratio photometry technique was used to check the method. The results indicated that Ca~(2+)_(c) at the bud, flowering, wilting and senescing stages were 8, 62, 12 and 15　nmol/L, respectively. When cut flowers were treated with ACC 1　mmol/L, Ca~(2+)_(c) rapidly increased to 108 nmol/L in conjunction with the premature opening of the flowers, and no change occurred in the value of Ca~(2+)_(c) during senescence. When the protoplasts were treated with IP_(3) 3.6 μmol/L, Ca~(2+)_(c) decreased, especially at the opening stage, but this kind of Ca~(2+) release could be inhibited by introducing La~(3+), a potent blocker of Ca~(2+) channel, into the cell, These results suggest that the opening of flowers can be induced by a rise of Ca~(2+)c from a relatively constant resting level of 10 nmol/L and IP_(3) can affect the Ca~(2+) channel activity in the plasma membrane.

Key words: ACC;Calcium holneostasis;IP_(3);Proaoplast Chinese;Rose petal

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Citing This Article：

Song Chunpeng, Cao Gengsheng, An Guoyong, Ma Xiaodong, Du Zuliang, Zhu Ziqiang, Yan Longfei. The effects of 1-aminocyclopropane-carboxylic acid and inositol-1,4,5-trisphosphate on cytoplasmic calcium concentration during Chinese rose petal development. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).