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The effect of mucilaginous substances extracted from foxtail millet embryogenic callus cultures on its protoplast cultures

Dong Jinjiang, Xia Zhenao

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences.Shanghai 200032

Abstract

Embryogenic callus cultures of cereal crops are usually accompanied by the excretion of mucilaginous substances, but the composition and function of these substances are yet to be analyzed. In this paper, the authors reported the isolation of protoplasts from embryogenic calli with mucilage by brief enzyme treatment,and the protoplast isolation was greatly facilitated by coupling moderate mechanic application. The authors added the mucilage extract from embryogenic callus of foxtail millet (Setaria italica L. Beauv.)to the media for culture of its protoplasts,to assess the effect of the extract on protoplast culture.The results showed that the addition of the extract promoted cell wall formation by protoplasts.Within the range of test,the number of the intact cells formed from protoplasts was correlated with the plating efficiency of protoplast culture,which was greatly enhanced by the addition of the mucilage extract to the protoplast culture media.

Key words: Foxtail millet;Protoplast culture;Plating efficiency.;Embryogenic callus;Mucilage extract

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Citing This Article：

Dong Jinjiang, Xia Zhenao. The effect of mucilaginous substances extracted from foxtail millet embryogenic callus cultures on its protoplast cultures. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).