Issue Archive

Study on cell suspension culture of Taxus yunnanensis

Gan Fanyuan, Zheng Guangzhi, Peng Liping, Luo Jianping

Kunming Institute of Botany, The Chinese Academy of Sciences. Kunming 650204, Yunnan

Abstract

B5 medium was suitable for cell suspension culture of Taxus yunnanensis. The growth cycle of suspension cells was about 30 days. The optimum size of inoculum and 2,4-D concentration for cell growth and taxol content was 0.5-0.8 g(cell DW)/100　ml medium and 1.0 mg/L respectively. Taxol content could be promoted by increasing the sucrose concentration to 40 g/L, and also by supplementing with organic substances like coconut milk(CM), caseinhydrolysat(CA) and lactalbumin hydrolysate(LH). Only CM and CA promoted cell growth of culture cells. Effects of different concentrations of NH_(4)NO_(3) on cell suspension culture of Taxus yunnanensis were not significant.

Key words: Taxus yunnanensis;Cell suspension;Culture taxol

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Gan Fanyuan, Zheng Guangzhi, Peng Liping, Luo Jianping. Study on cell suspension culture of Taxus yunnanensis. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).