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Localization of chloroplast CTK-binding protein in cells of wheat leaves

Zhang Huamin, Liu Yu, Wang Meiqi, Shen Yungang

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai 200032

Abstract

The localiation of CTK-binding protein in cells of wheat leaves was investigated using autoradiography and immuno-gold labelling technique. Electron microscope autoradiographs showed that silver grains (~(3)H-6BA) were localized only at the thylakoid membanes of chloroplasts. Immuno-gold localization showed that in wheat leaf cells, gold particles were mainly associated to chloroplasts, where they were concentrated in the thylakoid membranes. These facts suggest that the chloroplast CTK-binding protein is restricted to the thylakoid membranes of wheat chloroplasts.

Key words: Wheat leaf cells;CTK-binding protein;Autoradiography;Immuno-gold

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Citing This Article：

Zhang Huamin, Liu Yu, Wang Meiqi, Shen Yungang. Localization of chloroplast CTK-binding protein in cells of wheat leaves. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).