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Effects of cytochalasin B on stomatal movement of Vicia faba L.

Huang Rongfeng, Wang Xuechen

College of Biological Sciences, Beijing Agricultural University. Beijing 100094

Abstract

Effects of cytochalasin B (CB) on stomatal opening and closing of Vicia faba were investigated. The apertures of open or closed stomata were not significantly influenced by pretreatment with CB, a specific inhibitor of actin filament polymerization, at 10 mg/L for 50 min. However, stomatal movement was inhibited by CB and the inhibition could be reversed 3 h after CB was removed. Stomatal closure induced by ABA, Ca~(2+), or darkness was inhibited by pretreatment with CB 10 mg/L for 50 min in the light. Stomatal opening induced by K~(+) and light was partially inhibited by pretreatment with CB 10 mg/L for 50 min in the dark. These results suggest that microfilaments may mediate stomatal movement induced by Ca~(2+), ABA, darkness, K~(+) and light.

Key words: Microfilaments;Stomatal movement;Abscisic acid;Cytochalasin B

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Citing This Article：

Huang Rongfeng, Wang Xuechen. Effects of cytochalasin B on stomatal movement of Vicia faba L.. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).