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Effects of epibrassinolide on cell differentiation in Arabidopsis thaliana

Chen Jichu, Xu Madi, Zhao Yuju

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai 200032

Abstract

Arabidopsis thaliana have been used to investigate the role of epibrassinolide (epi-BR) in cell differentiation and regeneration in vitro. The results show that calli cultured on MS medium supplemented with 0.05, 0.5 or 5.0mg/L epi-BR formed much green buds and shoots. Supplementing the culture medium with epi-BR and 0.1mg/L KT induced the greening of callus and bud formation, but KT did not induce this differentiation alone. In addition, electron microscopic examination shows that normal chloroplasts were contained in cell of green callus cultured on medium with epi-BR.

Key words: Epibrassinolide (epi-BR);Arabidopsis thaliana;Callus;Cell differentiation

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Citing This Article：

Chen Jichu, Xu Madi, Zhao Yuju. Effects of epibrassinolide on cell differentiation in Arabidopsis thaliana. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).