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Effects of 5^ upstream region deletion on rice waxy gene expression

Yao Caiping, Wang Zongyang, Cai Xiuling, Zhang Dong, Zhang Jingliu, Hong Mengmin

Shanghai Institute of Plant Physiology, The Chinese Academy of Sciences. Shanghai 200032

Abstract

To identify the cis-acting element (s) within the 5^ upstream region of the rice waxy gene, a series of deletion mutants was constructed by using Exo III to digest the waxy promoter from 5^ end. Each of the chimeric genes consisting of these deleted sequences and coding sequence of beta-glucuronidase (GUS) gene was respectively introduced into rice protoplasts mediated by PEG and the expression of the chimeric gene was examined after incubation of these protoplasts for 48 h at 26 ℃. The luciferase (LUC) gene was co-introduced in each experiment, and its expression evaluated as an internal control. The data showed that the GUS activity decreased as the 5^ upstream region was shortened. When the waxy promoter was deleted from -861 bp to -640 bp, the GUS relative activity dramatically decreased. this result implicates that a cis-acting element located between -861 bp and -640 bp may play a regulatory role in the expression of the rice waxy gene.

Key words: Rice;Waxy gene;Cis-acting element;Transient expression

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Citing This Article：

Yao Caiping, Wang Zongyang, Cai Xiuling, Zhang Dong, Zhang Jingliu, Hong Mengmin. Effects of 5^ upstream region deletion on rice waxy gene expression. Acta Physiol Sin 1996; 48 (4): (in Chinese with English abstract).