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Study on the stimulative effect of sulfite on the activity of CF_(1)-ATPase in different states

He Xiangdong, Du Ziyun, Li Youze

Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032

Abstract

The effects of sulfite on CF_(1)-ATPase in different methods were studied. Sulfite stimulate stimulated the activity of heat/DTT-activated soluble and thylakoid membrane-bound CF_(1)-ATPase activity was weaker. The affinity of heat/DTT-activated membrane-bound CF_(1)-ATPase for sulfite and the maximal activity of heat/DTT-activated membrane-bound CF_(1)-ATPase stimulated by sulfite were lower than those of soluble CF_(1)-ATPase. In the presence of sulfite, the activation energy of thylakoid membrane-bound CF_(1)-ATPase was higher than that of soluble CF_(1)-ATPase. These results indicate that the catalytic function of CF_(1) is controlled by membrane-bound CF_(0). The reduction of disulfide bond in γ subunit could further increase the stimulative effect of sulfite on heat- or methanol-activated soluble and thylakoid-membrane-bound CF_(1)-ATPase. The stimulative effect of sulfite on the methanol-activated soluble and thylakoid membrane-bound CF_(1)-ATPase was dependent on the methanol concentration in the reaction medium. Its stimulative effect was more pronounced at sub-optimal methanol concentrations than at the optimum concentration. The relieving effect of sulfite on the inhibiton of the methanol-activated CF_(1)-ATPase activity by Mg~(2+) was different from that of the heat/DTT-activated CF_(1)-ATPase. Why sulfite has different effects in relieving the inhibition of the activity of differently activated CF_(1)-ATPase by Mg~(2+) needs further study.

Key words: Chloropldet H~(+)-ATPase;Heat activation;Heat/DTT activation;Methanol activation;Sulfite

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Citing This Article：

He Xiangdong, Du Ziyun, Li Youze. Study on the stimulative effect of sulfite on the activity of CF_(1)-ATPase in different states. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).