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Distribution and some properties of chloroplast chaperonin in some algae

Zhao Ruohong, Zhang Xiankong, Miao Yougang, Li Liren

Institute of Hydrobiology,The Chinese Academy of Sciences.Wuhan 430072.Hubei；China

Abstract

The analysis of Weatern blot with the antibody raised against to chloroplast chaperonin (cpn60) from pea showed that cpn60 occured in the eukaryotic green algae such as Chlamydomonas reinhardtii and prokaryotic nitrogen-fixing filamental cyanobacteria such as Anabaena siamensis could cross-react with the antibody to cpn60 from pea. The molecular weight of the #alpha#-subunit from the algae was about 60 kD, similar to that from pea; but the molecular weight of #beta#-subunit was distinctly greater than that from pea. The cpn60, however, could not be detected in unicellular cynobacteria such as Anacystis nidulans with the antibody to cpn60 from pea. The content of cpn60 in filamental cyanobacteria and green algae increased following heat shock, but decreased greatly when the algae were kept at -20℃ for 12 h. The electron-microscopic studies by using gold-labeled antibody raised against cpn60 from pea showed that the cpn60 in Anabaena siamensis occurred mainly in the vegetative cells, but not in the heterocysts.

Key words: Chloroplast chaperonin;Cyanobacteria;Green algae

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Citing This Article：

Zhao Ruohong, Zhang Xiankong, Miao Yougang, Li Liren. Distribution and some properties of chloroplast chaperonin in some algae. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).