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Preparation and application of monoclonal antibodies specific for abscisic acid methyl ester

Zhou Xie, Zheng Zhifu, Chen Puyan, Zhang Di

Departments of Agronomy,Nanjing Agricultural University.Nanjing 210095,Jiangsu；China；Nanjing Agricultural Institute.Nanjing 210046,Jiangsu

Abstract

A monoclonal antibody 5 E 11-D7-51, IgG_(1), which specifically recognizes abscisic acid methyl ester (ABAme) is described. This antibody was derived from an immunogen in which the carrier proteins were linked to ABA at 1-COOH. It showed very little cross-reactivity with ABA (1%) or ABA glucosyl ester (3.5%). Other compounds structurally similar to ABA, such as 2-cis-xanthoxin, violaxanthin and both the 2, trans-isomer and the (R)-enantiomer of ABA, were not found to exhibit any cross-reactivity (Table 1). Based on the use of this antibody, an enzyme-linked immunosorbent assay (ELISA) with high sensitivity and precision was developed, which displayed a linear range of 0.048-1.52 pmol of ABAme. Using this ELISE, as well as an established ELISA for gibberellins (GA_(1+3)), the levels of ABA and GA_(1+3) in senescing leaves of Rumex japonicus were examined. The results showed that the decrease in GA_(1+3)/ABA ratio was connected with the senescence of leaves of Rumex japonicus.

Key words: Abscisic acid (ABA);Gibberellin (GA);Monoclonal antibody (MAb);Enzyme-linked immunosorbent assay (ELISA);Rumex japonicus;Senescence

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Citing This Article：

Zhou Xie, Zheng Zhifu, Chen Puyan, Zhang Di. Preparation and application of monoclonal antibodies specific for abscisic acid methyl ester. Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).