Issue Archive

Relationship between the change of protein and the content of chlorophyll in the leaves of albescent line of wheat during the time when the plants were turning albescent

Guo AiGuang, Wang ZhenYi, Wang BaoLi, Wang Peihong

Northwetern Agricultural University.Yangling,Shaanxi 712100

Abstract

The mutant albescent line of wheat (Triticum aestivum L.cv Aibian No.1)is a special temporarily pigment-deficient mutant.It was found that the level of apoprotein was low in the albescent leaves.When the albescent leaves turned green,CP Ⅱappeared earlier than CPⅠ and the accumulation of chlorophyll seemed to be the prerequisite for the appearance of pigment-protein complexes. In addition, the increase in chlorophyll content showed positive cor-relation with the appearance of pigment-protein complexes,as well the content of proteins with MW 105,65,50,41 and 30 kD,but the content of proteins with 21 and 25kD remained high even in the completely albescent leaves.The SDS-PAGE results indicated that the levels of large subunits of Rubisco were lower than those in normal leaves. In contract,the level of small subunits of Rubisco was only slightly lower. With the increase in chlorophyll, protein synthesis rates increased sharply and the large subunits of Rubisco accumulated very quickly.These events partly resulted from the formation of thylakoid in chloroplast. Proteinase activity rose in albescent leaves, but declined in those turing green again.

Key words: Mutant albescent line of wheat;Pigmentprotein complex;Chlorophyll;Protein synthesis;Proteinase

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Guo AiGuang, Wang ZhenYi, Wang BaoLi, Wang Peihong. Relationship between the change of protein and the content of chlorophyll in the leaves of albescent line of wheat during the time when the plants were turning albescent. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).