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Comparison of the redox properties of right-side out and inside out plasmalemma vesicles purified from wheat roots

Qiu Quansheng, Liang Hougou, Jiao Xinzhi, Ni Jinshan

Laboratory of Physiology,Lanzhou University.Lanzhou 730001,Gausu；China

Abstract

Plasmalemma vesicles were purified from 8-day-old wheat (Triticum asetivum var.Longchun No.10) roots by discontinuous sucrose gradient centrifugation (SG) and aqueous two-phase partitioning (PP).Both IO and RO had high redox activities. The rates of NAD(P)H oxidation and Fe(CN)~(3)-_(6) reduction by IO was higher than that by RO while the EDTA-Fe~(3+) reduction of IO was lower than that RO.SHAM-stimulated NADH oxidation by IO was signifiicant higher than that by RO;But SHAM-stimulated O_(2) uptake by IO was only slightly higher than that by RO. And SHAM-stimulated NADH oxdation and O_(2) uptake by both IO and RO were completely inhibited by KCN.Both IO and RO generated O~(-)_(2) with an optimum pH value of 6.0.The O~(-)_(2) production by IO and RO was stimulated by SHAM and inhibited by SOD,catalase and KCN.

Key words: Plasmalemma;Right-side out vesicles;Inside out vesicles;Redox system;O_(2) generation;Wheatroots

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Citing This Article：

Qiu Quansheng, Liang Hougou, Jiao Xinzhi, Ni Jinshan. Comparison of the redox properties of right-side out and inside out plasmalemma vesicles purified from wheat roots. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).