Issue Archive

Properties of a CTK-binding protein from wheat chloroplasts

Zhang Huamin, Liu Yu, Shen Yungang

Shanghai Institute of Plant Physiology,The Chinese Academy of Sciences.Shanghai 200032

Abstract

A CTK-binding protein from wheat chloroplasts was shown to be heat-labile . It showed high binding activity to 6-BA by sedimentation method at 25℃ and pH 7-8. Scatchard plot showed two classes of binding sites in this CTK-binding protein, with Kα values of 1.1×10~(-9)mol/L and 9×10~(-7) mol/L. showed that the binding of 6-BA to this protein was partially inhibited by kinetin and zeatin, but not inhibited by ABA, GA, IAA and adenine. The amino acid composition of the CTK-binding protein indicated that it is rich in neutral amino acids and to have negative charge in a neutral medium.

Key words: Wheat chloroplast;CTK;CTK binding protein

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Zhang Huamin, Liu Yu, Shen Yungang. Properties of a CTK-binding protein from wheat chloroplasts. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).