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Response of two soybean cultivars with different drought resistance to exogenous H_(2)O_(2)

Xu Changcheng, Zou Qi, Fan Jilian, Zhao Shijie, Meng Qingwei

Plant Physiology and Biochemistry Laboratory,Shandong Agricultural University.Taian 271018,Shandong

Abstract

Leaf discs of a small-seed soybean cultivar Xiaolidou No. 1 (drought-resistant) and a commonly cultivated cultivar Ludou No. 4(drought-sensitive) were incubated in solutions containing different concentrations of H_(2)O_(2) for 12 h in light and in darkness.The activities of enzymes involved in the scavenging of active oxygen species were analyzed. Measurement of chlorophy11 contents in leaf discs showed a stronger resistance of Xiaolidou No. 1 than Ludou No. 4 to H_(2)O_(2) both in light and in darkness. The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were increased by sublethal concentrations of H_(2)O_(2) and were higher in Xiaolidou No. 1 than in Ludou No. 4. This increase in the activities of SOD,CAT and GR was inhibited by the protein synthesis inhibitor cycloheximide (CHM),but not by actinomycin (AMD). At higher concentrations of H_(2)O_(2) the activities of all these enzymes decreased, especially in Ludou No. 4. These results suggest that the induction of the activities of SOD,Cat and Rr in response to sublethal concentration of H_(2)O_(2) involves denovo protein synthesis, probably at the level of translation by long-life mRNA.

Key words: Exogenous hydrogen peroxide;Superoxide dismutase;Catalase;Glutathione reductase;Drought resistance;Soybean

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Citing This Article：

Xu Changcheng, Zou Qi, Fan Jilian, Zhao Shijie, Meng Qingwei. Response of two soybean cultivars with different drought resistance to exogenous H_(2)O_(2). Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).