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Changes in content of endogenous methyl jasmonate and gibberellins A_(3),A_(4) and A_(7) measured by gas chromtograph-mass spectrometry during stratification of apple Malus pumila l. seeds

Ma Huanpu, Blake P S, Browing G

Institute of Pomology,The Chinese Academy of Agricultural Sciences. Xingcheng 125100,Liaoning；UK

Abstract

Endogenous GA_(1),GA_(3),GA_(4)GA_(7) and methyl jasmonate (MeJA) were measured by GC-MS in cotyledon and embryonic axis of aplle seeds at each stage of stratification (5℃) and warm moist storage(17℃).The results showed that the percentage of seed germination and fresh weight of axes increased with time after 40 d of stratification. The contents of GA_(3) and GA_(7) also increased markedly at low temperature, a peak of GA_(3) was seen in the cotyledon and one of GA_(7) in the axis on 50th d of stratification No GA_(1) was detected at all stages of stratification, and no significant effect of low temperature on the level of GA_(4) was found in both cotyledon and axis. The pattern of MeJA change was not temperature-dependent but was evidently affected by high moisture. It can be suggested that GA_(3) and GA_(7) are involved in the release of dormancy by low temperature and there might be differences between GA_(3) and GA_(7).

Key words: Apple seed;Stratification;Low temperature;Gibberellins;Methyl jasmonate

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Citing This Article：

Ma Huanpu, Blake P S, Browing G. Changes in content of endogenous methyl jasmonate and gibberellins A_(3),A_(4) and A_(7) measured by gas chromtograph-mass spectrometry during stratification of apple Malus pumila l. seeds. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).