Issue Archive

Effects of TIBA,LaCl_(3),verapamil and TFP on auxin-induced tracheary differentiation and formation of bean hypocotyls

Li Huanxiu, Battey N

Sichuan Agricultural University.Yaan 625014,Sichuan

Abstract

Ten-day old bean seedlings (Phaseolus vulgaris L.) were treated with several chemical substances in the way shown in Fig. 1. After 5 days^ treatment, bean hypocotyls were cut into upper and lower parts at wound and 2. 0 cm above and below the wound. The two parts were put into freshly boiled lactic acid solutions (80%) for 3 d. Newly formed tracheas between 8 original vascular bundles were counted under microscope(×50). The results were as follows:The optimum NAA concentration for the induction of trachea formation was 10~(-4)mol/L. The key time for induction was the first 24 h. TIBA inhibited auxin-induced trachea formation in 72 h after wound formation. The maximum inhibition by TIBA occurred in the first 24 h. LaCl_(3) had inhibitive action on trachea only when used in the first 24 h, and Verapamil did so in 72 h after wound formation although both were Ca~(2+) channel blockers. TFP, a CaM antagonist, inhibited trachea formation only when used in the first 24 h. It was suggested that auxin transport and the presence of CaM and Ca~(2+) were important for trachea formation in bean hypocotyls. Trachea formation may begin at 24-48 h after wound formation.After 72 h,auxin-induced trachea formation ended, after which inhibitors did not affect auxin-induced trachea formatiom.

Key words: Auxin;Bean;Trachea differentiation and formation;Ca~(2+)channel blocker;CaM antagonist

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Li Huanxiu, Battey N. Effects of TIBA,LaCl_(3),verapamil and TFP on auxin-induced tracheary differentiation and formation of bean hypocotyls. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).