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Effects of AF64A on neurons containing both nitric oxide synthase and choline acetyltransferase in the rat septal complex

Yu Fulin, Shan Hongying, Dong Xinwen

Shanghai Institute of Physiology, Chinese Academy of Sciences. Shanghai 200031

Abstract

Ethylcholine mustard aziridinium ion(AF64A), a neurotoxic choline analog, was injected(ICV)bilaterally (1.5nmol/ventricle, n=10)into male adult rats to induce a model of Alzheimer^s disease(AD). One month later, using NADPH-diaphorase (NADPH-d) histochemistry followed by choline acetyltransferase(ChAT) immunocytochemistry(PAP)on the coronal sections of the septal complex, double-staining experiments were performed to assay the alterations of septalcholinergic neurons coexisted with nitric oxide synthase (NOS). These results indicate that: (1) the administration of AF64A has different effects on the cholinergic neurons with or without NOS in different subgroups of the septal complex,and the NADPH-d+ChAT double labelled neurons resist the neurotoxicity of AF64A; (2) in the intermediate subgroup, the cholinergic neurons containing NOS may have projections different from those without NOS.

Key words: AF64A;Nitric oxide pethase;NADPH-diaphorase histochemistry;Chorine acetylferase;Immunocpehemistry;Septal complex

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Citing This Article：

Yu Fulin, Shan Hongying, Dong Xinwen. Effects of AF64A on neurons containing both nitric oxide synthase and choline acetyltransferase in the rat septal complex. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).