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Frequency dependence of substance P release by electroacupuncture in rat spinal cord

Shen Shang, Bian Jingtan, Tian Jinbin, Han Jisheng

Neuroscience Research Center, Beijing Medical University. Beijing 100083

Abstract

In the present study we observed the frequency dependence of substance P (SP) release from rat spinal cord, with the frequencies of EA set at 2, 4, 8, 15, 30 and 100 Hz, The spinal perfusate was collected in 3 periods of 30 min before, during and after EA, and the immunoreactive SP (SP-ir)was measured by radioimmunoassay (RIA). The effectiveness of EA-induced analgesia was assessed by tail flick latency (TFL). Rats showing an increase of TFL over 40% was considered as EA responder. The results showed that in the responders, SP-ir in spinal perfusate showed a moderate decrease during 2 Hz EA, (P<0.01 compared with baseline level), no change in the 4 Hz EA group, and a marked increase during 8, 15, 30 and 100 Hz EA (P<0.01), with maximal increase occurring at 15 Hz(P<0.001).

Key words: Subonce P;Electroacupuncture;Radioimmunoassay;Spinal cord

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Citing This Article：

Shen Shang, Bian Jingtan, Tian Jinbin, Han Jisheng. Frequency dependence of substance P release by electroacupuncture in rat spinal cord. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).