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Tumor necrosis factor induced enhancement of neutrophil chemotaxis and adhesion through mediating of phospholipase A_(2) activation

Yan Guangtao, Hao Xiuhua, Li Zhenjia

Department of Biochemistry, Clinic Basic Institute of Medicine, Great Wall Hospital. Beijing 100853

Abstract

TNF(100ng/ml)given in vitro for 60 min, causes an obvious enhancement of neutrophil chemotaxis towards FMLP, and increased activity of adhesion toglass beads by stimulated release of TXB_(2) from PMN other than 6-keto-PGF_(1a). Similer neutrophil activation responces could also be promoted by in vitro addition of arachidonic acid (1 #mu#mol/ml)for 60 min. It was further found that PLA_(2)inhibitor, 4-bromophencyl bromide (5#mu#mol/ml), greatly inhibite the TNF induced responces, while cycloxgenase inhibitor indomethathin (85nmol/ml) had no effect on the activation of TNF.

Key words: Tumor necrosis factor;Neutrophil;Phosphonlipase A_(2);Chemotsxis;Adhesion; Arachidonic

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Citing This Article：

Yan Guangtao, Hao Xiuhua, Li Zhenjia. Tumor necrosis factor induced enhancement of neutrophil chemotaxis and adhesion through mediating of phospholipase A_(2) activation. Acta Physiol Sin 1995; 47 (6): (in Chinese with English abstract).