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The effect of GM-CSF/IL-3 on the grafting efficiency of CD34~(+) hemopoietic progenitor cells from unbilical cord blood

Pei Xuetao, Wu Zuze, Coutinho L H, Testa N G, Dexter T M

Beijing Institute of Radiation Medicine, Chinese Academy of Military Medicine. Beijing 100850

Abstract

CD34~(+) cells were isolated from human umbilical cord blood by using a high-gradient magnetic cell sorting system with anti-CD34 monoclonal antibody coated with microbeads, and then inoculated noto a pre-formed irradiated bone marrow stroma to evaluate the grafting efficiency of CD34~(+) cells with rhGM-CSF or IL-3, alone or in combination. The results showed that only 36% CD34~(+) cells in the control, and 68-89.6% in the groups with growth factors treatment seeded to the stroma. A short incubation of CD34~(+) cells with growth factors could rapidly reconstitute a long-term hemopoiesis in the long-term liquid culture system. This demonstrates that a brief treatment of CD34~(+) cells with GM-CSF or IL-3 can improve the grafting efficiency of transplanted umbilical cord blood cells.

Key words: Umbilical cord blood;CD34~(+) hematopoietic progenitor cells;Growth factors; Grafting efficiency

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Citing This Article：

Pei Xuetao, Wu Zuze, Coutinho L H, Testa N G, Dexter T M. The effect of GM-CSF/IL-3 on the grafting efficiency of CD34~(+) hemopoietic progenitor cells from unbilical cord blood. Acta Physiol Sin 1995; 47 (5): (in Chinese with English abstract).